화학공학소재연구정보센터
Current Microbiology, Vol.76, No.7, 818-823, 2019
Flavobacterium viscosus sp. nov. and Flavobacterium tangerina sp. nov., from Primates Feces
Strains YIM 102796(T) and YIM 102701-2(T) were isolated from the feces of Macaca mulatta and Hylobates hoolock, respectively, living in the Yunnan Wild Animal Park, Yunnan province of China. The two strains were Gram-stain-negative, non-gliding, produced flexirubin pigments, non-flagellated and aerobic bacteria. The 16S rRNA gene-based phylogenetic analysis indicate that both YIM 102796(T) and YIM 102701-2(T) are members of the genus Flavobacterium, closely related to F. ummariense DS-12(T) (95.9% similarity) and F. ceti 454-2(T) (93.8% similarity), respectively. The two strains shared 95.1% 16S rRNA gene sequence similarity. The average nucleotide identity and digital DNA-DNA hybridization values between the two strains were 76.5% and 22.9%, respectively, indicating that they are separate species. DNA G+Ccontents of YIM 102796(T) and YIM 102701-2(T) were 32.3mol% and 34.0mol%, respectively. Strains are able to grow at 4-37 degrees C, at pH 7.0-8.0 and in 0-2% (w/v) NaCl. Predominant fatty acid constituents (>7%) were iso-C-15:0, summed feature 3 (C(16:1)6c and/or C(16:1)7c) and summed feature 9 (iso-C(17:1)9c and/or 10-methylC(16:0)). Menaquinone 6 is major respiratory quinone. The predominant polar lipids were very similar to each other, comprising phosphatidylethanolamine, and multiple unknown aminolipids and unidentified polar lipids, and an unidentified aminophospholipid. On the basis of phenotypic and phylogenetic distinctiveness, it is suggested that the two strains represent two novel Flavobacterium species with strain YIM 102796(T) (=KCTC 52101(T)=CCTCCAB2016015(T)) as the type strain of Flavobacterium viscosus sp. nov. and strain YIM 102701-2(T) (=KCTC 52100(T)=CCTCCAB2016028(T)) as the type strain of Flavobacterium tangerina sp. nov.