화학공학소재연구정보센터
Journal of Hazardous Materials, Vol.368, 133-140, 2019
Rahnella sp. LRP3 induces phosphate precipitation of Cu (II) and its role in copper-contaminated soil remediation
Microbially induced phosphate precipitation (MIPP) is an advanced bioremediation technology to immobilize heavy metals in soil. In this study, an indigenous bacterial strain LRP3, identified as Rahnella sp., was isolated from Cu-contaminated dark brown soil in the mining area. Strain LRP3 could produce phytase and alkaline phosphatase to degrade phytic acid, which released soluble phosphate to the bacterial culture. Due to the metabolism of bacterial growth, the pH value of bacterial culture was increased. The minimum inhibitory concentration of Cu (II) to bacterial growth in solution was up to 130 mg/L. The bacterial culture could rapidly precipitate Cu (II) in solution through MIPP. The analysis results of Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDS), Fourier Transform Infrared Spectrometer (FTIR), and X-ray Diffraction (XRD) revealed that the precipitate form by bacterial culture was rod-shaped Cu-3(OH)(3)PO4 crystal with a diameter of 10 pm. The bacterial culture decreased the content of DTPA-Cu of 83 mg/kg soil in the soil by 58.2%, 61.5% and 75.8% after 5, 10 and 30 days of incubation, respectively, at the temperature of 25 degrees C. The results indicate that MIPP-based bioremediation by Rahnella sp. LRP3 is a practical, environmental friendly technology for the cleaning-up of copper-contaminated soil.