Industrial & Engineering Chemistry Research, Vol.58, No.8, 3555-3560, 2019
Catalytic Activity and Application of Immobilized Chloroperoxidase by Biometric Magnetic Nanoparticles
A novel immobilization method for chloroperoxidase (CPO) via carbohydrate-binding lectin protein, concanavalin A (ConA), was investigated. ConA was attached on the surface of silica-coated magnetic nanoparticles (MNPs) for effective recovery. CPO showed 100% activity recovery after its immobilization on ConA functionalized MNPs. After storing for four weeks, the immobilized CPO still maintained 80% activity while only 46% of activity of free enzyme was retained. Immobilized CPO by biometric magnetic nanoparticles exhibited excellent reusability and enantioselectivity in asymmetric synthesis of modafinil. More than 82% of original conversion rate and 70% enantiomeric excess (ee) value were observed after being run eight times. These results revealed that this immobilization strategy might have great potential in the application of other glycosyl-modified enzymes.