화학공학소재연구정보센터
Process Biochemistry, Vol.77, 18-22, 2019
Yield enhancement strategy of dithiolopyrrolone from Saccharothrix algeriensis by aliphatic alcohols supplementation
The production of dithiolopyrrolones by Saccharothrix algeriensis was investigated after supplementing the culture medium with ethanol and/or 1-butanol. Optimal conditions for the addition of ethanol to the culture medium provided a maximal dithiolopyrrolone titer of about 200 mg.L-1 after 5 days of culture, roughly corresponding to a 600%-increase. Using NAD(P)H oxidase inhibitor (diphenyleneiodonium) or reactive oxygen species scavenger (para-aminobenzoic acid), we suppose that ethanol promotes the formation of reactive oxygen species in Saccharothrix algeriensis, which, in turn, could induce biomass decline and dithiolopyrrolone overproduction. However, the underlying mechanisms remain to be elucidated. These results may be helpful for the control of dithiolopyrrolone yields from Saccharothrix algeriensis cultures.