화학공학소재연구정보센터
Journal of Applied Microbiology, Vol.125, No.6, 1786-1801, 2018
Rhizospheric bacterial isolates of grass pea (Lathyrus sativus L.) endowed with multiple plant growth promoting traits
Aim The main aim of this work was to examine the plant growth promoting, biocontrol activities and stress competency of grass pea rhizobacterial strains from Ethiopia. Methods and Results Serial dilutions were carried out to isolate rhizobacterial strains from the rhizosphere soil samples. The isolates were characterized for their plant growth promoting, biocontrolling and stress tolerance potential. The isolates that showed better performance in the tested parameters were identified by 16S rRNA gene sequencing. Among the isolates tested on Pikovskaya agar medium, 22 isolates that showed solubilization index of >2 center dot 41 cm were selected for further screening tests. Isolate AAUGPR-53 identified as Enterococcus species, Enterococcus casseliflavus strain showed the highest phosphate solubilization index and indole-3-acetic acid production efficiency of 4 center dot 81 +/- 0 center dot 02 (mu g ml(-1)) and 56 center dot 55 +/- 0 center dot 45 (mu g ml(-1)), respectively. Sixteen (72 center dot 7%) of the isolates showed in vitro antifungal inhibition against Fusarium oxysporum f. sp. lentis with isolates AAUGPR-92 and AAUGPR-91 identified as Enterococcus species, E. casseliflavus strain and Enterococcus gallinarum strain exhibiting the highest inhibition of 83 and 78%, respectively. Likewise, 68 center dot 2%, 91 center dot 30%, 45 center dot 5%, 77 center dot 3% and 100% of the isolates produced chitinase, protease, cellulase, HCN and NH3, respectively. Most of the isolates showed good tolerance to the tested stress factors. The 16S rRNA partial sequencing of the rhizobacterial isolates proved their taxonomic position in the existing bacterial isolates. Conclusion The results indicated that three strains, AAUGPR-53, 91 and 92, that showed maximum sequence identity (99%) to Enterococcus species, E. casseliflavus and E. gallinarum were recommended as microbial inoculants for trials under greenhouse and field conditions. Significance and Impact of the Study This study illustrates an effective alternative to conventional fertilizers that may contribute to crop disease reduction. Our results provide a foundation for future research that will lead to identifying potentially useful biocontrol strains found in the rhizosphere of grass pea.