화학공학소재연구정보센터
Process Biochemistry, Vol.73, 228-234, 2018
Ultrasound extraction of polysaccharides from guava leaves and their antioxidant and antiglycation activity
Plant polysaccharides possess multiple physiological activities. Box-Behnken design (BBD) was employed to optimize the ultrasound-assisted extraction of polysaccharides from guava leaves (GLP). It was found that ultrasonic time, temperature and power has a significant effect on the extraction efficiency. The yield of GLP was 1.00 +/- 0.04% under the optimal condition of extraction time of 20 min, temperature of 62 degrees C and ultrasound power of 404 W. The DPPH and ABTS(+) radical scavenging rate of GLP was 56.38% and 51.73% at 100 mu g/mL, respectively. The rate of inhibition of alpha-amylase by GLP was only 14.06% at 1 mg/mL; however, its a-glucosidase inhibition rate reached 99.54% at 100 mu g/mL. These results suggested that the ultrasound-assisted extraction for GLP is effective and environmentally friendly and GLP has wide application potentials as natural antioxidants and a-glucosidase inhibitors.