화학공학소재연구정보센터
Polymer(Korea), Vol.42, No.5, 866-873, September, 2018
근육유래 줄기세포의 평활근으로 분화유도를 위한 VEGF/Laminin 탑재 맴브레인
VEGF/Laminin Co-Immobilized Membrane for Enhanced Differentiation of Muscle-derived Stem Cells into Smooth Muscle Cells
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초록
본 연구에서는 생리활성인자[vascular endothelial growth factor(VEGF) 및 laminin]가 탑재된, 비대칭구조를 가지는 다공성 polycaporlactone(PCL) 기반의 맴브레인을 제조하였으며, 맴브레인에 탑재된 생리활성인자에 의한 근육유래 줄기세포(muscle-derived stem cells, MDSCs)의 평활근세포(smooth muscle cells, SMCs)로의 분화거동을 연구하였다. PCL 기반 맴브레인에 탑재된 VEGF 및 laminin은 맴브레인으로부터 각각 28일과 14일 동안 지속적으로 방출되었다. 각 생리활성인자가 단독 혹은 공동으로 탑재된 PCL 기반 맴브레인에서 MDSCs의 SMCs로의 분화거동을 DNA 정량화, RT-PCR 및 면역염색을 통해 분석하였으며, 두 생리활성인자가 공동으로 탑재된 PCL 기반 맴브레인에서 가장 우수한 평활근으로의 분화거동을 보임을 확인하였다. 이러한 결과로부터, VEGF/laminin이 탑재된, 비대 칭구조를 가지는 다공성 PCL 기반의 맴브레인은 평활근 재생을 위한 재생막으로 사용이 가능할 것이라 판단되었다.
It is important to control the stem cell differentiation into target cell types for clinical use of the stem cells. In this study, asymmetrically porous polycaprolactone (PCL)/Pluronic F127 membranes immobilized with bioactive molecules [vascular endothelial growth factor (VEGF) and/or laminin] were prepared to investigate the effect of musclederived stem cells (MDSCs) on smooth muscle cell (SMC) differentiation. The VEGF and laminin immobilized on the PCL/F127 membrane surface were released with a sustained manner for 28 and 14 days from the membrane, respectively. The SMC differentiation behavior of MDSCs on the membranes immobilized with single or dual bioactive molecules was compared by DNA quantification, RT-PCR, and immunohistochemical analyses. The dual VEGF/laminin-immobilized membrane group showed higher cell growth and more effective SMC differentiation than the single VEGF- or lamininimmobilized group. From our findings, we suggest that the dual VEGF/laminin-immobilized membrane may be applicable to use as a guided smooth muscle regeneration membrane.
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