화학공학소재연구정보센터
Macromolecular Research, Vol.26, No.8, 724-729, August, 2018
Evaluation of Saponin Loaded Gellan Gum Hydrogel Scaffold for Cartilage Regeneration
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Several concentrations of saponin (Sa), which is water-soluble, were fabricated well with gellan gum (GG) solution. Sa, found in many plants like clematis and ginseng, has long been used in traditional chinese medicine to treat joint diseases including osteoarthritis. Also it has its ability of anti-inflammatory, antioxidant and anti-cancer. GG hydrogel is suitable as a cell encapsulating agent in the field of cartilage regeneration because of its easy processing and biocompatibility. In this study, GG hydrogel scaffolds with different concentration of Sa were conducted for analysis by SEM, FT-IR, compressive strength, water uptake, degradation rate, MTT assay, mRNA expression. It was observed that 0.025 wt% Sa/GG hydrogel scaffold shows good morphology, cell proliferation and mRNA expression results. The composite material supports cell growth covered with extracellular matrix (ECM) with maintaining its function. As a result, incorporation of Sa loaded with GG hydrogel scaffolds had positive result up to 0.025 wt% in cartilage regeneration.
  1. Buckwalter JA, Martin JA, Adv. Drug Deliv. Rev., 58, 150 (2006)
  2. Krasnokutsky S, Samuels J, Abramson SB, Bull. NYU Hosp. Joint Dis., 65, 222 (2007)
  3. Felson D, Arthritis Res. Ther., 11, 203 (2009)
  4. Brooks PM, Curr. Opin. Rheumatol., 14, 573 (2002)
  5. Kurtz S, Ong K, Lau E, Mowat F, Halpern M, J. Bone Joint. Surg. Am., 89, 780 (2007)
  6. Kurtz SM, Lau E, Ong K, Zhao K, Kelly M, Bozic KJ, Clin. Orthop. Rel. Res, 467, 2606 (2009)
  7. Song JE, Song YS, Jeon SH, Choi IN, Kim CM, Khang GS, Polym. Korea, 41(4), 619 (2017)
  8. Qazi TH, Rai R, Boccaccini AR, Biomaterials, 35, 9068 (2014)
  9. Schumann D, Ekaputra AK, Lam CX, Hutmacher DW, Methods Mol. Med., 140, 101 (2007)
  10. Salgado AJ, Gomes ME, Chou A, Coutinho OP, Reis RL, Hutmacher DW, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 20, 27 (2002)
  11. Lee SJ, Int. J. Tissue Regen., 4, 89 (2013)
  12. Rucker M, Laschke MW, Junker D, Carvalho C, Schramm A, Mulhaupt R, Gellrich NC, Menger MD, Biomaterials, 27, 5027 (2006)
  13. Kim CM, Ha HJ, Kim HM, Kim EY, Song JE, Gao JQ, Khang G, Int. J. Tissue Regen., 5, 14 (2014)
  14. Cosgriff-Hernandez E, Mikos AG, Pharm. Res., 25, 2345 (2008)
  15. Li LM, Han M, Khang G, Gao JQ, Int. J. Tissue Regen., 4, 65 (2013)
  16. Ribeiro C, Correia V, Martins P, Gama F, Lanceros-Mendez S, Colloids Surf. B: Biointerfaces, 140, 430 (2016)
  17. Choi IN, Kim CH, Song JE, Baek JS, Jeon SH, Jeon HY, Lee SY, Khang GS, Polym. Korea, 41(6), 962 (2017)
  18. Wang C, Gong Y, Lin Y, Shen J, Wang DA, Acta Biomater., 4, 1226 (2008)
  19. Jin R, Teixeira LSM, Dijkstra PJ, van Blitterswijk CA, Karperien M, Feijen J, J. Control. Release, 152, 186 (2011)
  20. Balakrishnan B, Joshi N, Jayakrishnan A, Banerjee R, Acta Biomater., 10, 3650 (2014)
  21. Choi JH, Kim DY, Yoon JH, Youn HY, Yi J, Rhee HI, Ryu K, Jung K, Han CK, Kwak WJ, Osteoarthr. Cartil., 10, 471 (2002)
  22. Kim JH, Ryu KH, Jung KW, Han CK, Kwak WJ, Cho YB, J. Pharmacol. Sci., 98, 298 (2005)
  23. Lee SW, Chung WT, Choi SM, Kim KT, Yoo KS, Yoo YH, J. Ethnopharmacol., 101, 294 (2005)
  24. Li RW, Lin GD, Leach DN, Waterman PG, Myers SP, J. Ethnopharmacol., 104, 138 (2006)
  25. Siddiqi MH, Siddiqi MZ, Ahn S, Kang S, Kim YJ, Sathishkumar N, Yang U, Yang DC, J. Ginseng Res., 37, 261 (2013)
  26. Wu W, Xu X Dai Y, Xia L, Phytother. Res., 24, 538 (2010)
  27. Kareru P, Keriko J, Gachanja A, Kenji G, Afr. J. Tradit. Complement. Altern. Med., 5, 56 (2008)
  28. Vinay TN, Park CS, Kim HY, Jung SJ, Vet. Immunol. Immunopathol., 158, 73 (2014)
  29. Xu FT, Li HM, Zhao CY, Cell. Physiol. Biochem., 37, 1890 (2015)