화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.500, No.2, 224-228, 2018
Structural insights into the backbone-circularized granulocyte colony-stimulating factor containing a short connector
Backbone circularization is a powerful approach for enhancing the structural stability of polypeptides. Herein, we present the crystal structure of the circularized variant of the granulocyte colony-stimulating factor (G-CSF) in which the terminal helical region was circularized using a short, two-amino acid connector. The structure revealed that the N- and C-termini were indeed connected by a peptide bond. The local structure of the C-terminal region transited from an a helix to 310 helix with a bend close to the N-terminal region, indicating that the structural change offset the insufficient length of the connector. This is the first-ever report of a crystal structure of the backbone of a circularized protein. It will facilitate the development of backbone circularization methodology. (C) 2018 Elsevier Inc. All rights reserved.