화학공학소재연구정보센터
Applied Surface Science, Vol.451, 1-19, 2018
In vitro DNA binding, antioxidant, antimicrobial and anticancer assessment of amino acid functionalized magnetic nanoparticles
Biofunctionalized magnetic nanoparticles (FMNPs) were synthesized through in situ coprecipitations of Fe (II) and Fe (III) salts under air followed by the addition of amino acids (AA): serine (Ser), alanine (Ala), cysteine (Cys), histidine (His), and methionine (Met) via bottom-up approach without following any sophisticated route. However, the effect of amino acid side chain on the formation of gamma-Fe2O3 (maghemite) and Fe3O4 (magnetite) was attained through XRD, HR-TEM, SEM, VSM, FTIR, DLS and Mercury Porosimeter (MP). Surprisingly, our study depicts that except His; Ser, Ala, Cys and Met produced more than 50% of magnetite phase and inhibited the oxidation of Fe3O4 in the presence of air. Furthermore, AA@MNPs were used for in vitro biological assessment such as salmon testes DNA (STDNA) binding efficiency, DPPH center dot scavenging activity, antimicrobial and anticancer properties using UV-visible spectrophotometry and colorimeter respectively. The ST-DNA binding efficiency was observed at lambda(max) 259 nm and quantitative interaction was determined using 15-125 mM AA@MNPs and 500 mu g/ml DNA at physiological temperature, shows stacking interaction. The Cys@MNPs have exhibited highest DPPH center dot scavenging activity with SC50 2.5 mu g/mL at lambda(max) 525 nm. Antimicrobial property of AA@MNPs was studied on gram-positive (B. subtilis), gram-negative (E. coli) bacteria and fungi (C. albicans) using disc diffusion assay by measuring zone of inhibition (ZOI) in mm, mentioned as a diameter. Alongside, minimum inhibition concentration (MIC) was estimated by the dilution method for those strains sensitive against AA@MNPs. Although, AA@MNPs showed similar to 100% control growth of human lung cancer cell line (A549) with LC50, TGI and GI50 > 80 mu g/ml shown cells compatibility. These significant results revealed the impact of AA side chain with different surface activities of FMNPs. (C) 2018 Elsevier B. V. All rights reserved.