화학공학소재연구정보센터
Bioresource Technology, Vol.250, 505-512, 2018
Enrichment and adaptation yield high anammox conversion rates under low temperatures
This study compared two anammox sequencing batch reactors (SBR) for one year. SBRconstantT was kept at 30 degrees C while temperature in SBRloweringT was decreased step-wise from 30 degrees C to 20 degrees C and 15 degrees C followed by over 140 days at 12.5 degrees C and 10 degrees C. High retention of anammox bacteria (AnAOB) and minimization of competition with AnAOB were key. 5-L anoxic reactors with the same inoculum were fed synthetic influent containing 25.9 mg NH4+-N/L and 34.1 mg NO2--N/L (no COD). Specific ammonium removal rates continuously increased in SBRconstantT, reaching 785 mg NH4+-N/gVSS/d, and were maintained in SBRloweringT, reaching 82.2 and 91.8 mg NH4+-N/gVSS/d at 12.5 and 10 degrees C respectively. AnAOB enrichment (increasing hzsA and 16S rDNA gene concentrations) and adaptation (shift from Ca. Brocadia to Ca. Kuenenia in SBRloweringT) contributed to these high rates. Rapidly settling granules developed, with average diameters of 1.2 (SBRconstantT) and 1.6 mm (SBRloweringT). Results reinforce the potential of anammox for mainstream applications.