Bioresource Technology, Vol.251, 407-410, 2018
Consolidated bioprocessing for sodium gluconate production from cellulose using Penicillium oxalicum
The feasibility of consolidated bioprocessing for sodium gluconate production from cellulose was studied. A recombinant strain named z19 was constructed from Penicillium oxalicum wild-type strain 114-2 for simultaneous expression of glucose oxidase and catalase from Aspergillus niger. While keeping a cellulolytic ability similar with that of 114-2, z19 secreted certain amounts of glucose oxidase and catalase. Fed-batch and two-stage temperature control strategy (0-120 h, 30 degrees C; 120-192 h, 45 degrees C) was utilized for sodium gluconate production from cellulose (filter paper power), with 13.54 g/L of sodium gluconate obtained at the end of the fermentation. The results provide an alternative route for producing sodium gluconate from cellulose in a one-pot reaction.