Biochemical and Biophysical Research Communications, Vol.496, No.2, 253-259, 2018
Role of the mRNA export factor Sus1 in oxidative stress tolerance in Candida albicans
In eukaryotes, the nuclear export of mRNAs is essential for gene expression. However, little is known about the role of mRNA nuclear export in the important fungal pathogen, Candida albicans. In this study, we identified C albicans Susl, a nucleus-localized protein that is required for mRNA export. Interestingly, the sus1 Delta/Delta, displayed hyper-sensitivity to extracellular oxidative stress, enhanced ROS accumulation and severe oxidative stress-related cell death. More strikingly, although the mutant exhibited normal activation of the expression of oxidative stress response (OSR) genes, it had attenuated activity of ROS scavenging system, which may be attributed to the defect in OSR mRNA export in this mutant. In addition, the virulence of the sus1 Delta/Delta was seriously attenuated. Taken together, our findings provide evidence that the mRNA export factor Sus1 plays an important role in oxidative stress tolerance and pathogenesis. (C) 2018 Elsevier Inc. All rights reserved.