화학공학소재연구정보센터
Applied Chemistry for Engineering, Vol.28, No.6, 696-704, December, 2017
Lactobacillus pentosus에 의한 발효 전후 마가목 가지 추출물의 항산화 활성 및 Matrix Metalloproteinases 발현 억제 효과
Comparison of Antioxidant and Matrix Metalloproteinases Inhibitory Effects of Sorbus commixta Twig Extracts before and after Fermentation with Lactobacillus pentosus
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초록
본 연구에서는 마가목 가지 비발효 추출물과 발효균주 Lactobacillus pentosus를 이용하여 발효시킨 발효 추출물에 대하여 항산화 및 MMPs 발현 억제 효과를 조사하고 유효 성분을 분석하였다. 마가목 가지 비발효 추출물과 발효 추출물의 자유 라디칼 소거 활성(FSC50)에서 비발효 추출물은 41.0 μg/mL, 발효 추출물은 58.2 μg/mL이었다. Fe3+-EDTA/H2O2계에서 활성산소 소거 활성(OSC50)은 각각 2.6, 3.0 μg/mL로 나타났다. 진피 섬유아세포에서 세포내활성산소 소거활성은 10 μg/mL에서 각각 35.3, 40.2%를 나타났다. 진피 섬유아세포에서 MMPs (MMP-1, MMP-2 및 MMP-3) 발현은 10 μg/mL에서 비발효 추출물은 각각 68.3, 35.0 및 24.2%이었고, 발효 추출물은 각각 84.3, 70.5 및 69.2% 억제되었다. 마가목 발효 전후 추출물의 성분 변화는 TLC, HPLC 및 LC/ESI-MS/MS를 이용하였다. 그 결과, caffeic acid, (-)-epicatechin, isoquercitrin 및 quercetin을 확인하였다. 이상의 결과들은 L. pentosus를 이용한 마가목 가지 발효 추출물은 비발효 추출물보다도 ROS 소거 활성이 크게 나타났고 또한 MMPs 발현 억제 효능도 보여주었다. 따라서 마가목 가지 발효 추출물은 항노화 화장품 소재로서 응용 가능성이 있음을 시사하였다.
In this study, we investigated antioxidative and matrix metalloproteinases (MMPs) inhibitory effects of Sorbus commixta twig extracts and fermented extracts with Lactobacillus pentosus and analyzed active ingredients. The free radical scavenging activity (FSC50) of non-fermented and fermented extracts of S. commixta twig’ were 41.04 and 58.2 μg/mL, respectively. In the Fe3+-EDTA/H2O2 system, the active oxygen scavenging activity (OSC50) was 2.6 and 3.0 μg/mL, respectively. In the dermal fibroblasts, the intracellular reactive oxygen species (ROS) scavenging activity was 35.3% for non-fermented extract and 40.2% for fermented extracts at the concentration of 10 μg/mL. Also at the same concentration, the expression of MMPs (MMP-1, -2, -3) by western blot was 68.3, 35.0 and 24.2%, respectively for non-fermented extracts and 84.3, 70.5 and 69.2% for fermented extracts. TLC, HPLC, and LC/ESI-MS/MS were used for measuring the changes in the components of the extract before and after fermentation. As a result, caffeic acid, (-)-epicatechin, isoquercitrin, and quercetin were identified. From the results, S. commixta twig fermented extracts by L. pentosus showed greater ROS scavenging activity and inhibitory effects on MMPs expression than those of using non-fermented extracts. Therefore, it is suggested that S. commixta twig fermented extracts can be used as an anti-aging cosmetic material.
  1. Lee HS, Kim SH, J. Korean Soc. Food Sci. Nutr., 39, 1213 (2010)
  2. Gilchrest BA, J. Am. Acad. Dermatol., 21, 610 (1989)
  3. Zaid MA, Afaq F, Syed DN, Dreher M, Mukhtar H, Photochem. Photobiol., 83, 882 (2007)
  4. Kammeyer A, Luiten RM, Ageing Res. Rev., 21, 16 (2015)
  5. Kim M, Park YG, Lee HJ, Lim SJ, Nho CW, J. Agric. Food Chem., 63, 5428 (2015)
  6. Fisher GL, Talwar HS, Lin J, Lin P, McPhillips F, Wang Z, Li X, Wan Y, Kang S, Voorhees JJ, J. Clin. Invest., 101, 1432 (1998)
  7. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ, Nature, 379(6563), 335 (1996)
  8. Rittie L, Fisher GJ, Ageing Res. Rev., 1, 705 (2002)
  9. Park SG, Kim SN, Lee JC, Kim HS, Kim YJ, Lee BG, Jang IS, Korean J. Herbology., 19, 67 (2004)
  10. Im AR, Song JH, Lee MY, Yeon SH, Um KA, Chae S, BMC Complement. Altern. Med., 14, 424 (2014)
  11. Tsai CC, Chan CF, Huang WY, Lin JS, Chan P, Liu HY, Lin YS, Molecules, 18, 14161 (2013)
  12. Okabe Y, Shimazu T, Tanimoto H, J. Sci. Food Agric., 91, 658 (2011)
  13. Schmidt CG, Goncalves LM, Prietto L, Hackbart HS, Furlong EB, Food Chem., 146, 371 (2014)
  14. Park BG, Jung HJ, Cho YW, Lim HW, Lim CJ, J. Pharm. Pharmacol., 65, 457 (2013)
  15. Yang HJ, Kim EH, Park JO, Kim JE, Park SN, J. Soc. Cosmet. Sci. Korea, 35, 47 (2009)
  16. Ahn YJ, Won BR, Kang MK, Kim JH, Park SN, J. Soc. Cosmet. Sci. Korea, 35, 125 (2009)
  17. Yu T, Lee YJ, Jang HJ, Kim AR, Hong SY, Kim TW, MYK, Lee JH, Lee YG, Cho JY, J. Ethnopharmacol., 134, 493 (2011)
  18. Kim SH, Jang YS, Chung HG, Choi MS, Kim SC, Korean J. Plant. Res., 6, 120 (2003)
  19. Bhatt LR, Bae MS, Kim BM, Oh GS, Chai KY, Molecules, 14, 5323 (2009)
  20. Na MK, An RB, Lee SM, Min BS, Kim YH, Bae KH, Kang SS, Nat. Prod. Sci., 8, 26 (2002)
  21. Lim GN, Park MA, Park SN, J. Korean Oil Chem. Soc., 28, 482 (2011)
  22. Ridwan BU, Koning CJM, Besselink MGH, Timmerman HM, Brouwer EC, Verhoef J, Gooszen HG, Akkermans LMA, Lett. Appl. Microbiol., 46, 61 (2008)
  23. Quan LH, Cheng LQ, Kim HB, Kim JH, Son NR, Kim SY, Jin HO, Yang DC, J. Ginseng Res., 34, 288 (2010)
  24. Pyo YH, Lee TC, Lee YC, Food Res. Int., 38, 551 (2005)
  25. Min JW, Kim HJ, Joo KS, Kang HC, J. Soc. Cosmet. Sci. Korea, 41, 375 (2007)
  26. Kim SH, Min JW, Quan LH, Lee S, Yang DU, Yang DC, J. Ginseng. Res., 36, 291 (2012)
  27. Xuan SH, Park YM, Ha JH, Jeong YJ, Park SN, Pharmacol. Rep., Doi: 10.1016/j.pharep.2017.05.012 (2017).