화학공학소재연구정보센터
Biotechnology Progress, Vol.33, No.3, 726-736, 2017
Enrichment of Apoplastic Fluid with Therapeutic Recombinant Protein for Efficient Biofarming
Objective: For efficient biofarming we attempted to enrich plant interstitial fluid (IF)/apoplastic fluid with targeted recombinant therapeutic protein. We employed a synthetic human Glucocerebrosidase (GCB), a model biopharmaceutical protein gene in this study. Results: Twenty one Nicotiana varieties, species and hybrids were initially screened for individual IF recovery and based on the findings, we selected Nicotiana tabacum NN (S-9-6), Nicotiana tabacum nn (S-9-7) and Nicotiana benthamiana (S-6-6) as model plants for raising transgenic expressing GCB via Agrobacterium mediated transformation under the control of M24 promoter; GCB specific activity in each transgenic lines were analyzed and we observed higher concentration of recombinant GCB in IF of these transgenic lines (S-9-6, S-9-7, and S-6-6) in comparison to their concentration in crude leaf extracts. Conclusion: Recovery of valuable therapeutics in plant IF as shown in the present study holds great promise for promoting plant based biofarming. (C) 2017 American Institute of Chemical Engineers.