화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.487, No.2, 189-193, 2017
DOK1/PPARgamma pathway mediates anti-tumor ability of all-trans retinoic acid in breast cancer MCF-7 cells
Previous studies have showed the anticancer effect of the all-trans retinoic acid (ATRA) in many tumors including breast cancer; however, the underlying molecular mechanism is still poorly understood. This study experimentally revealed that ATRA treatment inhibited MCF-7 cell proliferation and promoted its apoptosis, along with an enhanced expression of docking protein 1 (DOK1). ATRA's effects on cell proliferation and apoptosis were prevented by DOK1 knockdown. In addition, the genetic silence of DOK1 can inhibit PPAR gamma expression and its activity. Moreover, inactivation of PPAR gamma by its specific inhibitor GW9662 reversed the impacts of ATRA on cell proliferation and apoptosis. Taken together, these results indicate that ATRA-enhanced expression of DOK1 activates PPAR gamma leading to inhibition of cell proliferation and enhancement of cell apoptosis in MCF-7 cell. (C) 2017 Elsevier Inc. All rights reserved.