화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.486, 124-129, 2017
Functional characterization of Brassica napus DNA topoisomerase I alpha-1 and its effect on flowering time when expressed in Arabidopsis thaliana
Previous studies have shown that DNA topoisomerase I alpha (AtTOP1 alpha) has specific developmental functions during growth and development in Arabidopsis thaliana. However, little is known about the roles of DNA topoisomerases in the closely related and commercially important plant, rapeseed (Brassica napus). Here, the full-length BnTOP1 alpha-1 coding sequence was cloned from the A2 subgenome of the Brassica napus inbred line L111. We determine that all BnTOP1 alpha paralogs showed differing patterns of expression in different organs of L111, and that when expressed in tobacco leaves as a fusion protein with green fluorescent protein, BnTOP1 alpha-1 localized to the nucleus. We further showed that ectopic expression of BnTOP1 alpha-1 in the A. thaliana top1 alpha-7 mutant fully complemented the early flowering phenotype of the mutant. Moreover, altered expression levels in top1 alpha-7 seedlings of several key genes controlling flowering time were restored to wild type levels by ectopic expression of BnTOP1 alpha-1. These results provide valuable insights into the roles of rapeseed DNA topoisomerases in flowering time, and provide a promising target for genetic manipulation of this commercially significant process in rapeseed. (C) 2017 Elsevier Inc. All lights reserved