화학공학소재연구정보센터
Applied Chemistry for Engineering, Vol.28, No.2, 198-205, April, 2017
Graviola (Annona muricata) 잎 추출물의 항산화 및 미백 효과
Antioxidant and Skin Whitening Effect of Graviola (Annona muricata) Leaf Extracts
E-mail:
초록
본 연구에서는 Annona muricata 잎 추출물(graviola leaf extracts, GLE)의 항산화(antioxidant), 항균(antibacterial), 미백 (whitening) 및 주름 개선(anti-wrinkle) 효과 등을 조사하였으며, 항산화(antioxidant) 효과에 대한 성은 전자공여능 분석을 통해 측정하였다. 그 결과 GLE는 농도 의존적으로 전자공여능이 증가하는 것으로 나타났다. GLE의 항균 활성은 paper disc법으로 측정하여 나타냈으며, 표준물질인 ampicillin과 비교하여 메티실린내성 황색포도상구균 CCARM3115 에서 비교적 높은 항균 활성을 나타내었다. GLE에 대한 미백(whitening) 효과 역시 tyrosinase 저해 활성 측정을 통해 진행하였으며, 그 결과 GLE는 농도가 증가함에 따라 tyrosinase 활성이 감소하는 것으로 나타났다. GLE 농도에 따른 L-tyrosine을 DOPA로 바꾸는 데 관여하는 hydroxylation 반응과 관련한 mushroom tyrosinase의 저해 활성은 표준물질인 arbutin에 비해 125∼250 μg/mL 농도 범위에서 특히 높게 나타났다. B16-F10 cell에 대한 melanin 생합성 저해능은 농도의존적으로 감소하는 경향을 보였으며, 100 μg/mL에서 76.7%를 나타났다. 주름 생성과 관련하여서는 elastase 저해 활성 측정을 통해 진행하였으며, 그 결과 동일한 농도에서 GLE 및 ursolic acid는 10.5, 56.5%로 나타났다. 이러한 결과를 통해 Annona muricata 잎 추출물(Graviola Leaf Extracts, GLE)이 항산화 및 미백 활성에 상당한 효과가 있음을 확인할 수 있었으며, 미백 화장품 성분으로서 과색소침착 치료에 유용할 것으로 기대된다.
In this study, graviola leaf extracts (GLE) was investigated for the effect of antioxidant, antibacterial, whitening, anti-wrinkle. The antioxidant effect of GLE was measured by an electron donating ability assay. As a result, GLE increased the electron donating ability in a concentration-dependent manner. The antibacterial effect of GLE was found to show the higher antibacterial effect in Methicillin-resistant Staphylococcus aureus CCARM3115 compared with that of ampicillin by a paper disc method. The whitening effect of GLE was also measured by tyrosinase inhibition assay, and it was found that the tyrosinase activity of GLE decreased as the concentration increased. The inhibition activity of tyrosinase involved in hydroxylation reaction which is related to converting L-tyrosine to (DOPA) was higher than that of arbutin’s at the concentration ranging from 125 to 250 μg/mL. In addition, GLE reduced melanin contents of B16-F10 melanoma cells in a dose-dependant manner and decreased to about 76.7% at a concentration of 100 μg/mL Regarding wrinkling formation of GLE, an elastase inhibition assay was performed. As a result, GLE and ursolic acid were 10.5% and 56.5%, respectively under the identical concentration. These results suggest that GLE has significant antioxidant and whitening activities, and also may be potentially used as a therapeutic agent for hyperpigmentation treatment as an ingredient of whitening cosmetics.
  1. Seo KS, Park JS, Go MY, Hwang SW, Jang JE, 2015 Cosmetics Industry Analysis Report, 6-7, Hanhak Munhak, Seoul, Korea (2016).
  2. Kim EH, Korean J. Aesthet. Cosmetol., 4, 195 (2006)
  3. Yuan SS, Chang HL, Chen HW, Yeh YT, Kao YH, Lin KH, Wu YC, Su JH, Life Sci., 72, 2853 (2003)
  4. N’Gouemo P, Koudogbo B, Tchivounda H, Nguema C, Etoua M, Phytother. Res., 11, 243 (1997)
  5. Carbajal D, Casaco A, Arruzazabala L, Gonzalez R, Fuentes V, J. Ethnopharmacol., 33, 21 (1991)
  6. Shirwaikar A, Rajendran K, Kumar C, Indian J. Exp. Biol., 42, 803 (2004)
  7. Marklund S, Marklund G, Eur. J. Biochem., 47, 469 (1974)
  8. Singleton VL, Rossi JA, Am. J. Enol. Vitic., 16, 144 (1965)
  9. Davis WB, Anal. Chem., 19, 476 (1947)
  10. Kim IH, Lee DG, Lee SH, Ha JM, Ha BJ, Kim SK, Lee JH, Biotechnol. Bioeng., 12, 579 (2007)
  11. Ishihara Y, Oka M, Tsunakawa M, Tomita K, Hatori M, Yamamoto H, Kamei H, Miyaki T, Konishi M, Oki T, J. Antibiot., 44, 25 (1991)
  12. Kong KH, Park SY, Hong MP, Cho SH, Comp. Biochem. Physiol. B, 125, 563 (2000)
  13. Choi SS, Noh HS, Cho SH, Kong KH, J. Pharm. Soc. Korea, 45, 522 (2001)
  14. Park SK, Hong SK, Kim HJ, Kim BY, Kim T, Kang JS, Kim D, Korean Chem. Eng. Res., 47(5), 553 (2009)
  15. Tobin D, Thody AJ, Exp. Dermatol., 3, 99 (1994)
  16. Bernadette E, Marianne P, Bernhard P, J. Am. Acad. Dermatol., 38, 45 (1998)
  17. Gupta AS, Webb RP, Holaday AS, Allen RD, Plant Physiol., 103, 1067 (1993)
  18. Lim JA, Chung TY, Cho EJ, Cancer Prev. Res., 17, 257 (2012)
  19. Choi BD, Jeon HS, Lee YS, Joo EY, Kim NW, Korean J. Food Sci. Technol., 42, 250 (2010)
  20. Park JA, J. Korean Soc. Beauty Art, 12, 189 (2011)
  21. Kwon TD, Choi SW, Lee SJ, Chung KW, Lee SC, Korean J. Physic. Educ., 3, 891 (2001)
  22. Lee YS, Joo EY, Kim NW, Korean J. Food Preserv., 13, 616 (2006)
  23. The Korean Nutrition Society, Phytonutrients to Save My Body, 18-24, Dulnyouk Publisher, Seoul, Korea (2013).
  24. Kim HJ, Lee KJ, Ma KH, Cho YH, Lee SY, Lee DJ, Chung JW, Korean J. Int. Agric., 27, 529 (2015)
  25. Lim HJ, Lee HJ, Lim MH, Korean J. Aesthet. Cosmetol., 13, 445 (2015)
  26. Zhishen J, Mengcheng T, Jianming W, Food Chem., 64, 555 (1999)
  27. Heim KE, Tagliaferro AR, Bobilya DJ, J. Nutr. Biochem., 13, 572 (2002)
  28. Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH, Lee BH, Korean J. Food Sci. Technol., 44, 337 (2012)
  29. Sundarrao K, Burrows I, Kuduk M, Yi YD, Chung MH, Suh NJ, Chang IM, Int. J. Parmacogn., 31, 3 (1993)
  30. Spritz RA, Hearing VJ, Adv. Hum. Genet., 22, 1 (1994)
  31. Lin JY, Fisher DE, Nature, 445, 843 (2007)
  32. Prota G, Prog. Clin. Biol. Res., 256, 101 (1988)
  33. Nakajima H, Wakabayashi Y, Wakamatsu K, Imokawa G, Phytother. Res., 25, 1398 (2011)
  34. Lee SK, Hong HK, Kim SJ, Kim YK, Song YS, Ha Y, Lee D, Khang G, Polym. Korea, 34(5), 398 (2010)
  35. Wang KH, Lin RD, Hsu FL, Huang YH, Chang HC, Huang CY, Lee MH, J. Ethnopharmacol., 106, 353 (2006)
  36. Brenneisen P, Sies H, Scharffetter-Kochanek K, Ann. N. Y. Acad. Sci., 973, 31 (2002)