화학공학소재연구정보센터
Journal of Supercritical Fluids, Vol.121, 19-31, 2017
Supercritical carbon dioxide extraction of antioxidants from Crocus sativus petals of saffron industry residues: Optimization using response surface methodology
Supercritical carbon dioxide extraction investigated as a green technology for the extraction of antioxidant compounds from Crocus sativus petals. A Box-Behnken design (BBD) was employed to optimize the extraction temperature (40-80 degrees C), extraction time (20-60min), and extraction pressure (90-180 bar). Optimization process using response surface methodology carried out and 3D response surfaces were plotted from the mathematical model. The optimum conditions of extraction were estimated to be temperature of 62 degrees C, time of 47 min and pressure of 164 bar. Extraction using these optimized conditions achieved the best total phenolic content (1423 mg/100 g), total flavonoid content (180 mg/100 g), total anthocyanin content (103.4 mg/100 g), 2, 2-diphenyl- 1-picryihydrazyl free radical-scavenging activity (74.5%) and ferric reducing/antioxidant power value (3.9 mM). The experimental values agreed with those predicted, thus representing fitness of the model employed and the success of BBD in optimizing the extraction conditions. Principal components analysis (PCA) provided a better understanding of interactions between characteristics of extracted antioxidants. (C) 2016 Elsevier B.V. All rights reserved.