화학공학소재연구정보센터
Journal of Structural Biology, Vol.197, No.2, 181-190, 2017
Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes
Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content and interpretability of tomograms. We discuss the potential for sub-volume averaging in highlighting and investigating specific processes in situ, focusing on microtubule structure and viral infection. We show that (i) in situ sub-volume averaging from single tomograms can guide and complement segmentation of biological features, (ii) the in situ determination of the structure of individual viruses is possible as they infect a cell, and (iii) novel, transient processes can be imaged with high levels of detail. (C) 2016 The Authors. Published by Elsevier Inc.