화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.481, No.1-2, 38-45, 2016
Cloning and expressions of peroxisome proliferator activated receptor alphal and alpha2 (PPAR alpha 1 and PPAR alpha 2) in loath (Misgurnus anguillicaudatus) and in response to different dietary fatty acids
Peroxisome proliferator activated receptor alphal and alpha2 (PPAR alpha 1 and PPAR alpha 2) were investigated in loath (Misgurnus anguillicaudatus) by RACE (rapid amplification of cDNA ends) and qPCR (real-time quantitative PCR) for the first time. The cDNA sequences of PPAR alpha 1 and PPAR alpha 2 were 2042bp and 2407bp, respectively encoding 467 and 465 amino acids. Sequence alignments of deduced amino acids showed significant homology between the two subtypes of PPAR alpha, indicating 70% identity. The two genes revealed sensible changes in transcriptions during early life stages of the loath, and the highest transcriptions of the two genes both appeared at some day after hatching. PPAR alpha 1 predominantly expressed in liver, while PPAR alpha 2 markedly expressed in heart. The expression regulation of PPAR alpha 1 and PPAR alpha 2 in response to dietary fatty acids was determined in livers of loaches fed with diets containing fish oil (FO group) and soybean oil (SO group) for 75 days. The expression level of PPAR alpha 1 in FO group was significantly higher than those in SO group (P < 0.01), while the expression level of PPAR alpha 2 in FO group was also significantly higher than those in SO group (P < 0.05). There was no significant difference in the expression level between PPAR alpha 1 and PPAR alpha 2 in SO group, whereas significant difference in FO group. These indicated that lipid resources could regulate the expressions of these two genes in the loath. Our results will provide opportunities to better understand the functional characterization of PPAR alpha 1 and PPAR alpha 2 in further studies. (C) 2016 Elsevier Inc. All rights reserved.