Biochemical and Biophysical Research Communications, Vol.480, No.4, 669-674, 2016
Muscle fiber-type conversion in the transgenic pigs with overexpression of PGC1 alpha gene in muscle
The peroxisome proliferator-activated receptor gamma, co-activator 1 alpha(PGC1 alpha) effectively induced the biosynthesis of the mitochondria and the energy metabolism, and also regulated the muscle fiber type shift. Overexpression of PGC1a gene in mice led to higher oxidative muscle fiber composition in muscle. However, no researches about the significant differences of muscle fiber phenotype in pigs after PGCl alpha overexpression had been reported. The composition of muscle fiber-types which were distinguished by four myosin heavy chain(MYHC) isoforms, can significantly affect the muscle functions. In our study, we generated the transgenic pigs to investigate the effect of overexpression of PGCla gene on muscle fiber-type conversion. The results showed that the number of oxidative muscle fiber(typel muscle fiber) was increased and the number of glycolytic muscle fiber(type2b muscle fiber) was decreased in the transgenic pigs. Furthermore, we found that PGCla overexpression up-regulated the expression of MYHC1 and MYHC2a and down-regulated the expression of MYHC2b.The analysis of genes expression demonstrated the main differentially expressed genes were MSTN, Myog and FOX01. In conclusion, the overexpression of PGCla gene can promote the glycolytic muscle fiber transform to the oxidative muscle fiber in pigs. (C) 2016 Elsevier Inc. All rights reserved.