Biochemical and Biophysical Research Communications, Vol.480, No.3, 296-301, 2016
ALTERED MERISTEM PROGRAM1 has conflicting effects on the tolerance to heat shock and symptom development after Pseudomonas syringae infection
An Arabidopsis thaliana ALTERED MERISTEM PROGRAM1 (AtAMP1), which encodes a putative glutamate carboxypeptidase, not only controls shoot apical meristem development, but also is involved in tolerance response to abiotic stresses. Here, we introduce a novel mutant; named amp1-32 that is a phenocopier to previously isolated different amp1 mutant alleles. Interestingly, tiny leaves were continuously developed at the bottom of pre-emerged leaves in the ampl-32. The amp1-32 mutant was less sensitive to heat shock treatment lasting for 3 h, whereas disease symptoms were severely developed in the mutant after Pseudomonas syringae infection. The mRNA levels of 171 genes were significantly altered in the mutant, as compared to wild-type plants. The transcription of genes involved in hormone signaling, post embryonic development, and shoot development were up-regulated in the amp1-32 mutant, whereas expression of genes related to responsiveness to pathogens and (in)organic matters, were decreased in the mutant. Taken together, perturbation of CK-and ABA-related events by AMP1 mutation caused aberrant development phenotype and conflicting responses against abiotic and biotic stresses in Arabidopsis. (C) 2016 Elsevier Inc. All rights reserved.