화학공학소재연구정보센터
Journal of Electroanalytical Chemistry, Vol.779, 86-91, 2016
Electrochemical microfluidic devices for evaluation of drug metabolism
An electrochemical drug metabolizing sensor device was fabricated with a patterned electrode of polycrystalline indium tin oxide (pITO) film and a poly(dimethylsiloxane) (PDMS) thin layer microchannel. The pITO film is known to be suitable as an electrode for detecting direct electron transfer (DET) from cytochrome P450 (CYP) without any surface modification. However, the ITO surface is hydrophilic and this causes the CYP to detach from it, resulting in poor repeatability. This manuscript describes the simultaneous electrochemical measurement of drug metabolizing reactions that is achieved by continuously supplying the pITO electrode surface with a small amount of microsome CYP. To achieve this, the microchannel was evaluated for its flow uniformity and reproducibility, and the shape and dimensions needed for good performance were determined by using a highly uniform nickel mold obtained by employing a polishing process after electroplated mold fabrication. A clearly separated dual flow was established with the microchannel. Using dual pITO working electrodes arranged in parallel (dual flow measurement) and subtraction process, the current from only the drug metabolism reaction was obtained by introducing drugs and microsome CYP at one pITO electrode and introducing only microsome CYP at the other pITO electrode. We also performed a kinetic analysis of the CYP activity in our electrochemical microfluidic device. (C) 2016 Elsevier B.V. All rights reserved.