화학공학소재연구정보센터
Applied Surface Science, Vol.388, 775-785, 2016
Improvement of the bio-functional properties of TiO2 nanotubes
Titanium oxide nanotubes with diameters from ca. 40-120 nm fabricated by the anodic oxidation of Ti at a constant voltage (10-28 V) were investigated to identify factors improving their bio-functional properties. Prepared substrates were subsequently annealed at 450 degrees C and 650 degrees C to obtain nanotubes having a crystalline structure, and were then examined by SEM, XRD, XPS, BET and contact angle measurement techniques. The thermally stabilized surfaces were subjected to bidirectional functionalization: by deposition of a thin layer of Ca-P and by loading with silver nanoparticles. Three factors were found to promote the proliferation of osteoblast (U2OS) cells: a larger nanotube diameter, a higher annealing temperature, and the presence of a thin Ca-P layer. Differentiation of these cells (by ALP test) was stimulated by a higher (650 degrees C) nanotube annealing temperature, but not by a thin Ca-P layer. The TiO2 nanotubesmodified samples exhibited noticeable antibacterial properties. Moreover, the additional deposition of Ag nanoparticles almost completely inhibited the survivability of S. epidermidis cells beyond 3 h of contact. In conclusion, TiO2 nanotubes-modified surfaces exhibit distinct bone forming ability and significant antibacterial properties, and can be easily functionalized by a thin Ca -P layer or nano-Ag deposition for further improvement of the above functionalities. (C) 2016 Elsevier B.V. All rights reserved.