화학공학소재연구정보센터
Polymer(Korea), Vol.40, No.6, 915-924, November, 2016
하이드록시아파타이트가 비율별로 첨가된 실크 피브로인 지지체에서 토끼 골수 유래 줄기세포의 골분화
Osteogenesis Differentiation of Rabbit Bone Marrow-mesenchymal Stem Cells in Silk Scaffold Loaded with Various Ratios of Hydroxyapatite
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초록
실크 피브로인은 좋은 기계적인 특성과 세포 호환성 때문에 인공 골 재료로써 널리 사용되어왔다. 최근 실크피브로인이 가진 취약성 때문에 실크 피브로인은 다양한 재료들이 첨가되어 실크 복합 지지체로서 연구되고 있다. 본 연구에서는 실크 피브로인의 단점을 보완하기 위해 하이드록시아파타이트/실크 복합 지지체를 제작하였다. 하이드록시아파타이트/실크 복합 지지체는 기계적/생물학적 특징을 확인하기 위해 SEM, EDS, 물 침투성, 다공도, 압축강도, MTT assay, ALP 활성도, mRNA 발현을 통해 연구되었다. 실험 결과, 하이드록시아파타이트/실크 복합 지지체는 골 조직 공학을 위한 지지체로서 뛰어난 특성을 가지며, 토끼 골수 유래 줄기세포로부터 골 형성을 유도하는 잠재성을 확인하였다.
Silk fibroin (SF) has been widely used as an artificial bone material due to the good mechanical properties and cell compatibility. Recently it have been studied as hybrid silk scaffold with the addition of various materials because it has vulnerability. In this study, we manufactured hydroxyapatite (HAp)/SF hybrid scaffolds to complement the disadvantage of the SF. HAp/SF hybrid scaffolds were measured to study the mechanical/biological characteristics through SEM, EDS, measurement of water uptake, porosity, compressive strength, MTT assay, ALP activity and mRNAs expression. These results showed HAp/SF hybrid scaffold has excellent properties as a scaffold for bone tissue engineering and potential for inducing osteogenesis from rabbit bone marrow-mesenchymal stem cells (rBMSCs).
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