Bioresource Technology, Vol.212, 334-337, 2016
On-site hydrolytic enzymes production from fungal co-cultivation of Bermuda grass and corn cob
Solid state fermentation (SSF) is used to produce industrial enzymes. The objective of this study was to use a co -culture of Aspergillus niger GS1 and Trichoderma reesei, grown on a mixture of Bermuda grass and corn cob to obtain fermented forage (FF) rich in hydrolytic enzymes, as a value added ingredient for animal feed. FPase, amylase and xylanase productivities (dry matter, DM) were 8.8, 181.4, and 42.1 U g (1) h (1), respectively (1 U = reducing sugars released min 1), after 12-16 h of SSF with C/N = 60. Cellulose, hemicellulose and lignin decreased 1.6-, 2.7- and 1.9 -fold (DM), respectively. In vitro ruminal and true digestibility of DM was improved 2.4- and 1.4 -fold. Ruminal digestion of FF reduced 1.32 -fold the acetate:propionate ratio, which may reduce the environmental impact of ruminants feeding. On -site hydrolytic enzymes productivity using SSF without enzymes extraction could be of economic potential for digestibility improvement in animal feed. (C) 2016 Elsevier Ltd. All rights reserved.