화학공학소재연구정보센터
Journal of Electroanalytical Chemistry, Vol.630, No.1-2, 63-68, 2009
Bioelectrocatalytic reactivity of myoglobin in layer-by-layer films assembled with triblock copolymer Pluronic F127
in this paper, electroactive protein myoglobin (Mb) was successfully assembled layer-by-layer with poly(ethylene oxide) loo-poly(propylene oxide)(65)-poly(ethylene oxicle)(100) triblock copolymer (Pluronic) into {Pluronic/Mb}(n) films on solid Surfaces. Quartz crystal microbalance (QCM) and cyclic voltammetry (CV) were used to monitor the film growth and characterize the films. The Mb in stable {Pluronic/Mb}(n) films exhibited a nearly reversible CV peak pair at -0.34 V vs. SCE for Mb heme Fe(III)/Fe(II) couple, and the films demonstrated good electrocatalytic responses to oxygen, hydrogen peroxide, and nitrite. Compared with cast Mb-Pluronic films, {Pluronic/Mb}(n) multilayer films showed higher fraction of electroactive protein, better controllability in film construction, and better bioelectrocatalytic performances, indicating the potential application of the films as a new type of biosensors based on the direct electrochemistry of redox proteins without using mediators. The driving forces for the assembly of {Pluronic/Mb}(n) films were also Studied and believed to be mainly the ion-dipole interaction between positively charged groups of Mb and electronegative ether oxygen groups of nonionic Pluronic. (C) 2009 Elsevier B.V. All rights reserved.