화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.468, No.1-2, 157-160, 2015
Amyloid-beta peptide increases cell surface localization of alpha 7 ACh receptor to protect neurons from amyloid beta-induced damage
Amyloid-beta peptide 1-42 (A beta(1-42)) reduced PC-12 cell viability in a concentration (1-10 mu M)- and treatment time (48-72 h)-dependent manner. Nicotine prevented A beta(1-42)-induced PC-12 cell death, but conversely, the alpha 7 ACh receptor antagonist alpha-bungarotoxin enhanced A beta(1-42)-induced cell toxicity. Extracellularly applied A beta(1-42) significantly increased cell surface localization of alpha 7 ACh receptor in PC-12 cells as compared with that for non-treated control cells. Cell surface localization of alpha 7 ACh receptor in the brain of 5xFAD mouse, an animal model of Alzheimer's disease (AD), apparently increased in an age (1-12 months)-dependent manner in association with increased accumulation of A beta(1-42) in the plasma membrane component. Taken together, these results indicate that A beta(1-42) promotes translocation of alpha 7 ACh receptor towards the cell surface and that alpha 7 ACh receptor rescues neuronal cells from A beta(1-42)-induced damage. (C) 2015 Elsevier Inc. All rights reserved.