Molecular Crystals and Liquid Crystals, Vol.508, 191-199, 2009
In Vitro Skin Permeation Enhancement of Asiaticoside by Liquid Crystal Vesicles
Egg phosphatidylcholine (Egg PC) liposomal suspensions of Centella asiatica (C. asiatica) extract were prepared by a sonication method. On transmission electron microphotographs (TEM), multi-lamellar vesicles coexisted with small uni-lamellar ones. The size on TEM fell within the range of size distribution, obtained by a dynamic light scattering technique. In vitro permeation through hairless mouse skin was investigated using a diffusion cell. Asiaticoside, one of biological active ingredients of C. asiatica extract, was detected in the receptor cell. As controls, suspensions of C. asiatica extract in phosphate buffered saline (pH 7.4), 30% ethanol solution and absolute ethanol were investigated for the in vitro permeation of asiaticoside. The egg PC liposomes turned out to enhance the permeation of asiaticoside, compared with the control vehicles. Egg PC liposomes could be a candidate vehicles for the use as a permeation enhancer of asiaticoside.