화학공학소재연구정보센터
Protein Expression and Purification, Vol.113, 63-71, 2015
A novel method for expression and purification of authentic amyloid-beta with and without N-15 labels
Amyloid-beta (A beta) is a major constituent in the senile plaques of patients with Alzheimer's disease (AD). A beta has been intensively studied in amyloid research; however, challenges posed by data reproducibility arise from purity of synthetic A beta and high expense for its isotope-labeling. The difficulties motivate development and optimization of recombinant A beta (rA beta) production. Here, we report a new procedure to express and purify high quality rA beta 40 from Escherichia coil. The new A beta construct expressed insoluble A beta fused with an N-terminal histidine-tag connected by a linker harboring TEV protease cut site. After purification and partial refolding, the fusion tag was removed by TEV protease cleavage, immobilized metal affinity chromatography (IMAC), and reversed phase-HPLC purification with a yield of 3.5 mg/L culture with and without N-15 label. The rA beta adopts classic amyloid fibrillization and is capable of binding to its clinical relevant metal ions. (C) 2015 Elsevier Inc. All rights reserved.