화학공학소재연구정보센터
Applied Chemistry, Vol.3, No.2, 92-95, October, 1999
교반배양조건에서 Acetobacter xylinum의 응집에 의한 미생물셀룰로오스의 감소된 생산 - 주사전지혐미경에 의한 관찰
Reduced Production of Microbial Cellulose caused by Aggregation of Acebacter xylinum under Shaking Culture Conditions - Observation by Scanning Electron Microscope
Microbial cellulose has many potential applications due to its excellent physical properties. The production of cellulose by Acetobacter xylinum in submerged culture is, however, beset with numerous problems. The most difficult one has been the appearance of negative mutants under shaking culture conditions, which is deficient of cellulose procucing ability. In this paper, microbial cellulose produced under static culture conditions and shaking culture conditions were examined under scanning electron microscope. Microbial cells producing cellulose under shaking culture conditions aggregated, which is different from those under static culture conditions. The aggregation caused by shaking was accelerated by the fibrils of microbial cellulose due to their sticky characteristics and this might be the one of reasons why shaking culture generates much less or no cellulose than static culture.