화학공학소재연구정보센터
Polymer(Korea), Vol.40, No.2, 306-312, March, 2016
전기방사를 이용한 N-Carboxyethyl Chitosan/Poly(ethylene oxide) 조성의 나노섬유 제조와 유착방지제로의 활용
Preparation of N-Carboxyethyl Chitosan/Poly(ethylene oxide) Nanofiber Using Electrospinning and Its Application as Anti-adhesive
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초록
N-carboxyethyl chitosan(CECH)과 poly(ethylene oxide)(PEO)를 전기 방사용 용액으로 제조하고, 전기방사하여 얻은 나노섬유 지지체를 유착방지제로 응용하였다. Chitosan(CH)이 CECH로 합성된 것을 FTIR을 통해 관찰하였다. FE-SEM으로 CECH와 PEO의 농도별 나노섬유의 굵기를 측정하였고, 세포독성 평가와 동물실험을 진행하였다. CECH:PEO의 조성이 3:3일 때 전기방사하여 얻은 섬유가 가장 균일하고 고른 나노섬유 지지체를 형성하는 것을 SEM으로 확인하였다. CECH와 PEO, 그리고 전기방사하여 얻은 나노섬유 지지체는 모든 농도에서 90% 이상의 세포 생존율을 보였다. 유착수술 7일 후 CECH/PEO 나노섬유 지지체는 복강 내 유착 방지에 효과적임을 육안적 방법과 조직학적 방법을 통해 관찰하였다. In vivo 실험과 조직학적 시험을 통해 CECH와 PEO로 전기 방사하여 얻은 나노섬유 지지체의 유착방지제로의 활용 가능성을 확인하였다.
The nanofiber sheets consisted of N-carboxyethyl chitosan (CECH) and poly(ethylene oxide) (PEO) were developed using an electrospinning process to prevent abdominal adhesions after surgical operations. Successful synthesis of CECH from chitosan was confirmed by FTIR. The morphology and biocompatibility of the nanofiber sheets werecharacterized using a scanning electron microscope and MTT assay. For in vivo assessments, CECH/PEO nanofiber sheets were implanted into the abdominal surgical site and the abdominal tissues were evaluated by histological examination. When the weight ratio of CECH to PEO was 3:3, the nanofiber showed the most similar thickness between them. The MTT assay showed that the nanofibers had no significant toxicity toward NIH/3T3 cells at the high elution concentrations. The macroscopic and histological observation after 7 days of surgical process demonstrated that the CECH/PEO nanofiber sheets prevented abdominal adhesion effectively. In conclusion, the CECH/PEO nanofiber sheets can be exploited to prevent postsurgical adhesions.
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