화학공학소재연구정보센터
International Journal of Hydrogen Energy, Vol.39, No.32, 18235-18246, 2014
Comparative transcriptional study on the hydrogen evolution of marine microalga Tetraselmis subcordiformis
Its the first comparative transcriptome study of Tetraselmis subcordiformis' H-2 production with carbonyl cyanide m-chlorophenylhydrazone (CCCP) by de nova RNA-seq. The global analysis of the annotated genes and pathways unveiled it a good candidate for biofuels. Focusing on H-2 production, the genes coding active center subunit (HydA) and assembly subunits (HydEF and HydG) of hydrogenase proportionally expressed during the induction but stopped after illuminated; on contrast, the gene of the active centre stabilization subunit (Hyd3) highly expressed during the H-2 evolution, indicating its key role in the prolonged H-2 production. The results also showed genes of HCP4, ppGpp phosphohydrolase and RpoD relating to the hydrogenase expression regulation. On the energy aspect, genes of CF-ATPases and ferredoxin-NADP reductase in chloroplast down-regulated while Mito-ATPase in mitochondria and pyruvate metabolism pathway up-regulated during the H-2 production, consisting with our previous reports on physiological level. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.