화학공학소재연구정보센터
Journal of Electroanalytical Chemistry, Vol.374, No.1-2, 71-79, 1994
A Critical-Evaluation of a Glucose Biosensor Made by Codeposition of Palladium and Glucose-Oxidase on Glassy-Carbon
The preparation of a glucose biosensor by the electrochemical codeposition of palladium and glucose oxidase (GOx) on a glassy carbon electrode is described. The Pd/GOx electrode system allowed a low working potential of +0.3 V vs. AgAgCl which is ca. 0.3 V lower than that reported for equivalent Pt/GOx systems. The sensor required no special pretreatment to suppress interference from galactose. It is demonstrated that the pH of the plating solution has a critical effect on the successful codeposition of palladium and GOx. In addition, the results of a systematic study demonstrated that the deposition time as well as the concentration of the palladium salt and the enzyme level strongly affect the sensitivity of the resulting electrode. During a test period of 50 days the electrode lost approximately 50% of its sensitivity within the first 20 days, after which the response was essentially constant. The morphology of the electrode surface was characterized by scanning electron microscopy.