화학공학소재연구정보센터
International Journal of Hydrogen Energy, Vol.35, No.19, 10639-10644, 2010
High biohydrogen yielding Clostridium sp. DMHC-10 isolated from sludge of distillery waste treatment plant
A mesophilic high hydrogen producing strain DMHC-10 was isolated from a lab scale anaerobic reactor being operated on distillery wastewater for hydrogen production. DMHC-10 was identified as Clostridium sp. on the basis of 16S rRNA gene sequencing. Various medium components (carbon and nitrogen sources) and environmental factors (initial pH, temperature of incubation) were optimized for hydrogen production by Clostridium sp. DMHC-10. The strain, in late exponential growth phase, showed maximum hydrogen production (3.35 mol-H-2 mol(-1) glucose utilized) at 37 degrees C, pH 5.0 in a medium supplemented with organic nitrogen source. Butyric acid to acetic acid ratio was ca. 2.3. Hydrogen production declined when organic nitrogen was replaced with inorganic nitrogen. (C) 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.