화학공학소재연구정보센터
Applied Chemistry, Vol.6, No.1, 5-8, May, 2002
동맥 벽의 표적을 위한 유전자 전달 시스템
The Gene Delivery System to Target Arterial Wall Cells
In this study, we developed a specific cell-targeting gene delivery system with cationic polymer as non-viral vector. Artery wall binding peptide (AWBP), a specific targeting peptide, was conjugated to poly(ethylene glycol)-grafted-poly(L-lysine) (PEG-g-PLL) to enhance the transfection efficiency to artery wall cells. Charaacters of the physical and biochemical properties of the synthesized AWBP-PEG-PLL and AWBP-PEG-PLL/p DNA complex were analyzed through 1H-NMR, gel electrophoresis, atomic force microscopy (AFM) and from both bovine aorta endothelial cells (EC) and smooth muscle cells (SMC). We expected that AWBP-PEG-PLL, a gene delivery system for arterial wall targeting, will be used for genetic treatment of cardiovascular diseases. After this study, it will be continuously investigated for the synthesis of gene carrier using low molecular weight water soluble chitosan (LMWSC).