화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.448, No.1, 45-49, 2014
Soni-removal of nucleic acids from inclusion bodies
Inclusion bodies (lBs) are commonly formed in Escherichia coil due to over expression of recombinant proteins in non-native state. Isolation, denaturation and refolding of these IBs is generally performed to obtain functional protein. However, during this process IBs tend to form non-specific interactions with sheared nucleic acids from the genome, thus getting carried over into downstream processes. This may hinder the refolding of IBs into their native state. To circumvent this, we demonstrate a methodology termed soni-removal which involves disruption of nucleic acid-inclusion body interaction using sonication; followed by solvent based separation. As opposed to conventional techniques that use enzymes and column-based separations, soni-removal is a cost effective alternative for complete elimination of buried and/or strongly bound short nucleic acid contaminants from lBs. (C) 2014 The Authors. Published by Elsevier Inc.