Biochemical and Biophysical Research Communications, Vol.446, No.3, 720-725, 2014
Short-term effects of 7-ketocholesterol on human adipose tissue mesenchymal stem cells in vitro
Oxysterols comprise a very heterogeneous group derived from cholesterol through enzymatic and nonenzymatic oxidation. Among them, 7-ketocholesterol (7-KC) is one of the most important. It has potent effects in cell death processes, including cytoxicity and apoptosis induction. Mesenchymal stem cells (MSCs) are multipotent cells characterized by self-renewal and cellular differentiation capabilities. Very little is known about the effects of oxysterols in MSCs. Here, we describe the short-term cytotoxic effect of 7-ketocholesterol on MSCs derived from human adipose tissue. MSCs were isolated from adipose tissue obtained from two young, healthy women. After 24 h incubation with 7-KC, mitochondrial hyperpolarization was observed, followed by a slight increase in the level of apoptosis and changes in actin organization. Finally, the IC50 of 7-KC was higher in these cells than has been observed or described in other normal or cancer cell lines. (C) 2014 Elsevier Inc. All rights reserved.