화학공학소재연구정보센터
Journal of the Korean Industrial and Engineering Chemistry, Vol.7, No.5, 1020-1026, October, 1996
4차 암모늄기를 가진 키토산 유도체의 항균성
Antibacterial Activity by Chitosan Derivatives with Quaternary Ammonium Salt
초록
천연고분자인 chitin의 탈아세틸화물인 chitosan의 유리아미노기에 알킬 길이가 다른 알데히드를 이용하여 N-alkyl chitosan 유도체를 각각 합성한 다음, 이들을 methyl iodide로 4차화시킴으로서 4차 암모늄기가 도입된 새로운 수용성 chitosan 유도체를 합성하여, 도입된 알킬기의 길이에 따른 항균성을 그람양성균인 S. aureus(ATCC 6538P)와 그람 음성균인 E. coli(ATCC 14339)에 대해서 shake flask법을 이용하여 검토하였다. 그 결과 소수성이 증대될수록 항균력은 증가하였고, 특히 그람음성균인 E. coli 비해 그람양성균인 S. aureus에 대한 항균력이 더욱 증대됨을 확인할 수 있었다. 이는 cytoplasmic membrane의 구조상의 차이에서 기인한 것으로 생각된다.
Chitosan is the deacetylation product of chitin. Chitosan was converted to N-alkyl chitosan derivatives using proper aldehyde having different alkyl chains, then chitosan derivatives with quaternary ammonium salt were producted by quaternization with methyl iodide. Their antibacterial activities aganst S. aureus(ATCC 6538P) and E. coli(ATCC 14339), gram-positive bacteria and gram-negative bacteria, respectively, as introduced alkyl length were evaluated by shake flask method using colony count. The antibacteiral activity was found to be increased as alkyl chains, hydrophobic goups. These antibacterial agents were more active against S. aureus than E. coli. It may be due to the different of structure of cytoplasmic membrane.
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