Applied Surface Science, Vol.292, 764-772, 2014
Modified composite microspheres of hydroxyapatite and poly(lactide-co-glycolide) as an injectable scaffold
The compound of hydroxyapatite-poly( lactide-co-glycolide) (HA-PLGA) was prepared by ionic bond between HA and PLGA. HA-PLGA was more stable than the simple physical blend of hydroxyapatite and poly(lactide-co-glycolide) (HA/PLGA). The surface of HA-PLGA microsphere fabricated by an emulsion-solvent evaporation method was rougher than that of HA/PLGA microspheres. Moreover, surface HA content of HA-PLGA microspheres was more than that of HA/PLGA microspheres. In vitro mouse OCT-1 osteoblast-like cell culture results showed that the HA-PLGA microspheres clearly promoted osteoblast attachment, proliferation and alkaline phosphatase activity. It was considered that surface rich HA component and rough surface of HA-PLGA microsphere enhanced cell growth and differentiation. The good cell affinity of the HA-PLGA microspheres indicated that they could be used as an injectable scaffold for bone tissue engineering. (C) 2013 Elsevier B.V. All rights reserved.
Keywords:Hydroxyapatite;Poly(lactide-co-glycolide);Composite microspheres;Injectable scaffold;Tissue engineering