화학공학소재연구정보센터
Korea-Australia Rheology Journal, Vol.26, No.2, 209-215, May, 2014
Following-up changes in red blood cell deformability and membrane stability in the presence of PTFE graft implanted into the femoral artery in a canine model
E-mail:
It is known that a moderate mechanical stress can even improve the red blood cells’ (RBC) micro-rheological characteristics, however, a more significant stress causes deterioration in the deformability. In this study, we aimed to investigate the effect of the presence of artificial graft on the RBC deformability and membrane stability in beagles. In the Control group only anesthesia was induced and in the postoperative (p.o.) period blood samplings were carried out. In the Grafted group under general anesthesia, the left femoral artery was isolated, from which a 3.5 cm segment was resected and a PTFE graft (O.D.: 3 mm) of equal in length was implanted into the gap. On the 1st, 3rd, 5th, 7th and 14th p.o. days blood was collected the cephalic veins and RBC deformability was determined ektacytometry (LoRRca MaxSis Osmoscan). Membrane stability test consisted of two deformability measurements before and after the cells were being exposed to mechanical stress (60 or 100 Pa for 300 seconds). Compared to the Control group and the baseline values the red blood cell deformability showed significant deterioration on the 3rd, 5th and mainly on the 7th postoperative day after the graft implantation. The membrane stability of erythrocyte revealed marked inter-group difference on the 3rd, 5th and 7th day: in the Grafted group the deformability decreased and during the membrane stability test smaller difference was observed between the states before and after shearing. We concluded that the presence of a PTFE graft in the femoral artery may cause changes in RBC deformability in the first p.o. week. RBC membrane stability investigation shows a lower elongation index profile for the grafted group and a narrowed alteration in the deformability curves due to mechanical stress.
  1. Alexy T, Baskurt OK, Nemeth N, Uyuklu M, Wenby RB, Meiselman HJ, Biorheology, 48, 173 (2011)
  2. Arwatz G, Smits AJ, Biorheology, 50, 45 (2013)
  3. Baskurt OK, Handbook of Hemorheology and Hemodynamics, IOS Press, Amsterdam, 170-190 (2007)
  4. Baskurt OK, Boynard M, Cokelet GC, Connes P, Cooke BM, Forconi S, Hardeman MR, Jung F, Liao F, Meiselman HJ, Nash G, Nemeth N, Neu B, Sandhagen B, Shin S, Thurston G, Wautier JL, Clin. Hemorheol. Microcirc., 42, 75 (2009)
  5. Baskurt OK, Hardeman MR, Uyuklu M, Ulker P, Cengiz M, Nemeth N, Shin S, Alexy T, Meiselman HJ, Scand. J. Clin. Lab. Invest., 69, 777 (2009)
  6. Baskurt OK, Meiselman HJ, Clin. Hemorheol. Microcirc., 55, 55 (2013)
  7. Blackshear PL, Blackshear GL, Handbook of Bioengineering, McGraw-Hill, New York, 15.1-15.19 (1987)
  8. Chiu JJ, Chien SS, Physiol. Rev., 90, 327 (2011)
  9. Chiu JJ, Usami S, Chien S, Ann. Med., 41, 19 (2009)
  10. Esquivel CO, Blaisdell FW, J. Surg. Res., 41, 1 (1986)
  11. Fleser PS, Nuthakki VK, Malinzak LE, Callahan RE, Seymour ML, Reynolds MM, Merz SI, Meyerhoff ME, Bendick PJ, Zelenock GB, Shanley CJ, J. Vasc. Surg., 40, 803 (2004)
  12. Hardeman MR, Goedhart PT, Shin S, Handbook of Hemorheology and Hemodynamics, IOS Press, Amsterdam, 242-266 (2007)
  13. Hirayama T, Yamaguchi H, Allers M, Roberts D, William-Olsson G, Scand. J. Thorac. Cardiovasc. Surg., 19, 257 (1985)
  14. Johnson CA, Vandenberghe S, Daly AR, Woolley JR, Snyder ST, Verkaik JE, Ye SH, Borovetz HS, Antaki JF, Wearden PD, Kameneva MV, Wagner WR, Artif. Organs., 35, 9 (2011)
  15. Jung F, Braune S, Lendlein A, Clin. Hemorheol. Microcirc., 53, 97 (2013)
  16. Kameneva MV, Antaki JF, Handbook of Hemorheology and Hemodynamics, IOS Press, Amsterdam, 206-227 (2007)
  17. Koppensteiner R, Moritz A, Schlick W, Fenzl G, Roedler S, Ehringer H, Wollner E, Am. J. Cardiol., 67, 79 (1991)
  18. McGah PM, Leotta DF, Beach KW, Zierler RE, Riley JJ, Aliseda A, J. Vasc. Surg., 56, 403 (2012)
  19. Menu P, Stoltz JF, Kerdjoudj H, Clin. Hemorheol. Microcirc., 53, 117 (2013)
  20. Meram E, Yilmaz BD, Bas C, Atac N, Yalcin O, Meiselman HJ, Baskurt OK, Biorheology, 50, 165 (2013)
  21. de Oliveira S, Saldanha C, Clin. Hemorheol. Microcirc., 44, 63 (2010)
  22. Rutherford R, Vascular surgery, 5th ed., Saunders, Philadelphia, 719-725 (2000)
  23. Papp J, Toth A, Sandor B, Kiss R, Rabai M, Kenyeres P, Juricskay I, Kesmarky G, Szabados S, Toth K, Clin. Hemorheol. Microcirc., 49, 331 (2011)
  24. Sakota D, Sakamoto R, Sobajima H, Yokoyama N, Waguri S, Ohuchi K, Takatani S, Artif. Organs., 32, 785 (2008)
  25. Tatterton M, Wilshaw SP, Ingham E, Vanniasinkam SH, Vasc. Endovascular Surg., 46, 212 (2012)
  26. Ulker P, Sati L, Ozenci CC, Meiselman HJ, Baskurt OK, Biorheology, 46, 121 (2009)
  27. Wan J, Ristenpart WD, Stone HA, Proc. Natl. Acad. Sci. USA, 105, 16432 (2008)
  28. Watanabe N, Sakota D, Ohuchi K, Takatani S, Artif. Organs., 31, 352 (2007)