Applied Biochemistry and Biotechnology, Vol.170, No.3, 541-551, 2013
Effect of Bovine Serum Albumin (BSA) on Enzymatic Cellulose Hydrolysis
Bovine serum albumin (BSA) was added to filter paper during the hydrolysis of cellulase. Adding BSA before the addition of the cellulase enhances enzyme activity in the solution, thereby increasing the conversion rate of cellulose. After 48 h of BSA treatment, the BSA adsorption quantities are 3.3, 4.6, 7.8, 17.2, and 28.3 mg/g substrate, each with different initial BSA concentration treatments at 50 A degrees C; in addition, more cellulase was adsorbed onto the filter paper at 50 A degrees C compared with 35 A degrees C. After 48 h of hydrolysis, the free-enzyme activity could not be measured without the BSA treatment, whereas the remaining activity of the filter paper activity was approximately 41 % when treated with 1.0 mg/mL BSA. Even after 96 h of hydrolysis, 25 % still remained. Meanwhile, after 48 h of incubation without substrate, the remaining enzyme activities were increased 20.7 % (from 43.7 to 52.7 %) and 94.8 % (from 23.3 to 45.5 %) at 35 and 50 A degrees C, respectively. Moreover, the effect of the BSA was more obvious at 35 A degrees C compared with 50 A degrees C. When using 15 filter paper cellulase units per gram substrate cellulase loading at 50 A degrees C, the cellulose conversion was increased from 75 % (without BSA treatment) to a parts per thousand yen90 % when using BSA dosages between 0.1 and 1.5 mg/mL. Overall, these results suggest that there are promising strategies for BSA treatment in the reduction of enzyme requirements during the hydrolysis of cellulose.