화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.424, No.3, 508-511, 2012
Crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose
Starch branching enzyme (SBE) catalyzes the cleavage of alpha-1,4-linkages and the subsequent transfer of alpha-1,4 glucan to form an a-1,6 branch point in amylopectin. We determined the crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose at a resolution of 2.2 angstrom. Maltopentaose bound to a hydrophobic pocket formed by the N-terminal helix, carbohydrate-binding module 48 (CBM48), and a-amylase domain. In addition, glucose moieties could be observed at molecular surfaces on the N-terminal helix (alpha 2) and CBM48. Amino acid residues involved in the carbohydrate bindings are highly conserved in other SBEs, suggesting their generally conserved role in substrate binding for SBEs. (C) 2012 Elsevier Inc. All rights reserved.