The first analogues of LNA (Locked Nucleic Acids): Phosphorothioate-LNA and 2′-thio-LNA

https://doi.org/10.1016/S0960-894X(98)00366-7Get rights and content

Abstract

LNA (Locked Nucleic Acids, 1, X  O, Y  O) is a novel oligonucleotide analogue capable of recognizing complementary DNA and RNA with unprecedented thermal affinities. Synthesis of the first chemically modified LNA analogues is reported. A 9-mer phosphorothioate- LNA containing three LNA thymine monomers (1, X  O, Y  S, Base = thymin-1-yl) and 9-mer LNAs containing one, three or five 2′-thio-LNA monomers (1, X  S, Y  O, Base = uracil-1-yl) were able to recognize both complementary DNA and RNA with thermal affinities comparable to those of parent LNA.

Center for Synthetic Bioorganic Chemistry, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark

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