Abstract
Silymarin (Sm) from the fruit of Silybum marianum is an isomeric mixture of pharmacologically active flavonolignans which are formed by oxidative coupling of taxifolin (Tx) and coniferyl alcohol (CA). Suspension cultures of this plant constitutively secrete small amounts of Sm into the extracellular medium. Production can be increased by inclusion of cyclodextrins (CDs) in cultures. Both hydroxylated (RHCD) and dimethylated (RMCD) CDs strongly induced prompt accumulation of CA in the medium followed by a late production of flavonolignans. Simultaneous addition of methyl jasmonate (MJ) and RMCD to cells did not significantly modify CA release or flavonolignan accumulation. Delayed addition of MJ to cultures subcultivated in medium containing RMCD markedly influenced Sm production by promoting conversion of the previously formed CA precursor.
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Acknowledgments
This work was financed by Ministerio de Ciencia e Innovación (BFU2008-02876/BFI), and by the Consejería de Educación, Ciencia e Investigación de la Región de Murcia (BIO BVA 07 01 0003), Spain. We are grateful to Dr. C. Raposo, from the Spectroscopy Service of the University of Salamanca, for his valuable help at identifying CA and Sm compounds.
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Belchi-Navarro, S., Pedreño, M.A. & Corchete, P. Methyl jasmonate increases silymarin production in Silybum marianum (L.) Gaernt cell cultures treated with β-cyclodextrins. Biotechnol Lett 33, 179–184 (2011). https://doi.org/10.1007/s10529-010-0406-6
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DOI: https://doi.org/10.1007/s10529-010-0406-6