Type II collagen-chondroitin sulfate-hyaluronan scaffold cross-linked by genipin for cartilage tissue engineering
Section snippets
Collagen II sponge fabrication and cross-linking
COL II made from bovine trachea was prepared using previously defined protocols (19). A sample of 100 mg lyophilized collagen thus made was homogenized in 0.5 M acetic acid at 4 °C to give a final COL II concentration of 1% (wt/vol). The well-mixed slurry was poured into molds and then frozen and lyophilized at − 20 °C and − 58 °C, respectively. The fabricated COL II sponges were immersed overnight in 70% alcohol to remove acetic acid before cross-linking. CS with MW of ca. 100,000 from shark
Three-dimensional composite scaffold fabrication
The scaffolds used for tissue engineering must have high porosity with a large surface area/volume ratio to provide a greater space for cells and to allow the production of new ECM. In this study, porous COL II sponges were fabricated, both with and without CS and HA, using high purity type-II atelocollagen and cross-linker genipin to mimic the native ECM of articular cartilage. The best cross-linker for primary amino groups is the naturally existing reagent genipin, which has been reported to
Acknowledgments
This research is supported by the Ministry of Economic Affairs, Taiwan, under the Technology Development Program for Academia grant 91-EC-17-A-17-S1-0009.
References (26)
- et al.
Crosslinked type II collagen matrices: preparation, characterization, and potential for cartilage engineering
Biomaterials
(2002) - et al.
Tapping mode atomic force microscopy of hyaluronan: extended and intramolecularly interacting chains
Biophys J.
(1998) - et al.
Preparation and characterization of porous cross-linked collagenous matrices containing bioavailable chondroitin sulphate
Biomaterials
(1999) - et al.
Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide cross-linking
Biomaterials
(2002) - et al.
Development of tailor-made collagen-glycosaminglycan matrices: EDC/NHS crosslinking, and ultrastructural aspects
Biomaterials
(2000) - et al.
Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor
Biomaterials
(2005) - et al.
Chondrogenesis in a cell-polymer-bioreactor system
Exp. Cell Res.
(1998) - et al.
Effect of the controlled-released TGF-beta 1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold
Biomaterials
(2004) - et al.
Cartilage repair using new polysaccharidic biomaterials: macroscopic, histological, and biochemical approaches in a rat model of cartilage defect
Osteoarthr. Cartil.
(2003) - et al.
Gelatin-chondroitin-hyaluronan tri-copolymer scaffold for cartilage tissue engineering
Biomaterials
(2003)
In vitro surface characterization of a biological patch fixed with a naturally occurring cross-linking agent
Biomaterials
Effects of chondroitin sulphate and interleukin-1 on human articular chondrocytes cultivated in clusters
Osteoarthr. Cartil.
Markedly different effects of hyaluronic acid and chondroitin sulfate-A on the differentiation of human articular chondrocytes in micromass and 3-D honeycomb rotation cultures
J. Biomed. Mater. Res.
Cited by (111)
Effects of self-assembled type II collagen fibrils on the morphology and growth of pre-chondrogenic ATDC5 cells
2024, Osteoarthritis and Cartilage OpenBiomaterials and tissue engineering approaches using glycosaminoglycans for tissue repair: Lessons learned from the native extracellular matrix
2023, Acta BiomaterialiaCitation Excerpt :reported sponges containing gelatin-CS-HA in poly (lactic-co-glycolic acid) (PLGA) improved chondrogenic differentiation of MSCs and MSC/TGF-β3 immobilized sponges regenerated chondral defects in a rabbit model [172]. Similarly collagen-based HA and CS containing hydrogels also showed improved chondrogenic activity [173]. Chitosan and HA copolymers have also been extensively studied for cartilage tissue engineering [174].
Application of graphene in articular cartilage tissue engineering and chondrogenic differentiation
2023, Journal of Drug Delivery Science and TechnologyCollagen type II: From biosynthesis to advanced biomaterials for cartilage engineering
2021, Biomaterials and BiosystemsIn the quest of the optimal chondrichthyan for the development of collagen sponges for articular cartilage
2021, Journal of Science: Advanced Materials and DevicesCitation Excerpt :These observations not only confirm that the pore size and porosity were suitable for cell and ECM migration within the three-dimensional conformation of the scaffolds, but also indicate the chondrogenic potential of the scaffolds. Again these observations are in agreement with previous work in the field, where Jellyfish (R. esculentum) collagen sponges with pore size of 40–200 μm (at the centre of the scaffold, the pore diameter was 74 ± 18 μm and at superficial zones, the pore diameter was 50 ± 20 μm) [14] and bovine collagen type II sponges with pore size of 140 ± 30 μm [50] maintained chondrocyte phenotype and Jellyfish (R. esculentum) collagen sponges with porosity of 98.2 ± 0.4% induced chondrogenic differentiation of human mesenchymal stem cells [16]. With respect to gene analysis, in general all collagen scaffolds showed a higher chondro-inductive potential compared to TCP, as evidenced by downregulated expression of COL1A1 and COL3A1 (except the LSD scaffold) and upregulated expression of COL10A1, SOX9, COMP and ACAN (except the CR scaffold).