Biochemical and Biophysical Research Communications
Identification and characterization of a full-length cDNA encoding paramyosin of Trichinella spiralis
Section snippets
Materials and methods
Parasites and antigen preparation. T. spiralis (ISS533) was maintained in female ICR mice. Adult worm and muscle larvae were recovered from infected mice by the standard pepsin digestion method as described [11] and infection sera were collected. Crude somatic extracts of adult worms, muscle larvae, and excretory–secretory (ES) products were prepared by conventional methods [12].
Mice. Female BALB/c mice aged 6–8 weeks were obtained from the Laboratory Animal Center of the Academy of Military
Molecular characterization of the cDNA encoding paramyosin
After immunoscreening of a cDNA expression library (200,000 plaques) with rabbit antisera against T. spiralis adult extracts and T. spiralis-infected rabbit sera, the longest cDNA fragment was sequenced and its amino acid sequence was deduced. The full length of cDNA was 2996 bp. The ORF of the cDNA consisted of 2655 bp encoding 885 amino acids with a predicted molecular mass of 102 kDa and an isoelectric point of 5.4. The cDNA consisted of a 5′-untranslated region of 74 bp and a 3′-untranslated
Acknowledgments
We thank Fengyun Wang and Jin Pan for their technical assistance. This work was supported by grants from the National Natural Science Foundation of China (30571626), the Natural Science Foundation of Beijing (5062005), the Beijing Municipal project for Developing Advanced Human Resources for Education (BAHED), and the PhD Programs Foundation of the Ministry of Education of China (20060025003).
References (24)
- et al.
Clinical, haematological, biochemical and economic impacts of Trichinella spiralis infection in pigs
Vet. Parasitol.
(2007) - et al.
The epidemiology of human trichinellosis in China during 2000–2003
Acta Trop.
(2006) - et al.
Molecular cloning of a cDNA encoding a putative cuticle collagen of Trichinella spiralis
Vet. Parasitol.
(2005) - et al.
Influence of adjuvant formulation on the induced protection of mice immunized with total soluble antigen of Trichinella spiralis
Vet. Parasitol.
(2005) - et al.
Secretion of the novel Trichinella protein TSJ5 by T. spiralis and T. pseudospiralis muscle larvae
Mol. Biochem. Parasitol.
(2001) - et al.
Update on paramyosin in parasitic worms
Parasitol. Int.
(2005) - et al.
Mapping of the complement C9 binding domain in paramyosin of the blood fluke Schistosoma mansoni
Int. J. Parasitol.
(2007) - et al.
The vaccine efficacy of native paramyosin (Sj-97) against Chinese Schistosoma japonicum
Int. J. Parasitol.
(1998) - et al.
A vaccine against the Asian schistosome, Schistosoma japonicum: an update on paramyosin as a target of protective immunity
Int. J. Parasitol.
(1997) - et al.
Paragonimus westermani: biochemical and immunological characterizations of paramyosin
Exp. Parasitol.
(2007)