Biochemical and Biophysical Research Communications
Platelet-rich plasma stimulates osteoblastic differentiation in the presence of BMPs
Section snippets
Materials and methods
Materials. Bovine plasma was purchased from Funakoshi, Tokyo, Japan. Dulbecco’s modified Eagle’s medium (DMEM) α-minimal essential medium (α-MEM) were obtained from Sigma–Aldrich (St. Louis, MO). Fetal bovine serum (FBS) and calf serum (CS) were obtained from Moregate Biotech (Bulimba, Qld, Australia). Bovine thrombin was obtained from Wako Pure Chemical Industries (Osaka, Japan). Recombinant BMP-2 produced in Escherichia coli was obtained from Acris Antibodies GmbH (Nordhein-Westfalen,
PRP and soluble fraction of PRP stimulate BMP-4-induced osteoblastic differentiation and proliferation of C2C12 myoblasts
BMP-4 at 20 ng/ml slightly induced ALP activity in C2C12 cells (Fig. 1A). Addition of human PRP to the cultures stimulated ALP activity at lower concentrations, although the degree of stimulation gradually decreased at higher concentrations (Fig. 1A). In contrast, human PPP failed to stimulate ALP activity at all concentrations examined (Fig. 1A). We further examined the stimulatory activity in the soluble fractions prepared from bovine activated PRP and PPP. The ALP activity induced by BMP-4 at
Discussion
In the present study, we found that the soluble fraction prepared from activated PRP stimulates osteoblastic differentiation in the presence of BMPs in mesenchymal progenitor cells. Pretreatment of scaffolds with BMP-4 and the soluble PRP fraction stimulated osteoblastic differentiation in vitro. Because PRP is an autologous blood product, simultaneous application of PRP and BMPs with scaffolds may be a simple and useful method for enhancement of bone formation in vivo.
The stimulatory activity
Acknowledgments
This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan, and in particular by a Ministry Grant to the Saitama Medical University Research Center for Genomic Medicine.
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