Nutritional enrichment of larval fish feed with thraustochytrid producing polyunsaturated fatty acids and xanthophylls
Section snippets
Microorganism and culture media
Schizochytrium sp. strain KH105 (31) was maintained on an agar medium containing 30 g/l glucose, 10 g/l Polypepton, 5 g/l yeast extract, and 15 g/l agar in 19.5 g/l artificial sea salts (about 50% equivalent of natural seawater; Jamarine Laboratory, Osaka). The seed culture medium consisted of 50 ml of 30 g/l glucose, 20 g/l Polypepton and 10 g/l yeast extract in 50% artificial seawater. For optimization, cultivation was performed in 200-ml baffled flasks containing 50 ml of culture medium
Optimization of culture condition of Schizochytrium sp. KH105
To make use of the Schizochytrium cells as enrichment diet material, we aimed first to optimize the medium composition for the cultivation of the strain KH105 to improve the cellular lipid content. Figure 1A shows the effect of salt concentrations ranging from 10% to 100% of that of natural seawater in the basal medium on the cell growth and production of fatty acids and DHA. These parameters were maximum at a seawater concentration of 50%, but did not change significantly when the salt
Acknowledgments
The authors thank the Sasebo City Fisheries Center for preparing the rotifers.
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